Background: Burn injuries represent a significant global health burden, with grade II burns causing substantial morbidity and healthcare costs. Aloe barbadensis Miller (Aloe vera) has been traditionally used for wound healing, but scientific evidence for its efficacy in burn wound management remains limited.

Objective: This study investigated the therapeutic effects of Aloe barbadensis Miller ethanol extract on grade II burn wound healing in Wistar rats, evaluating its potential as an alternative treatment modality.

Methods: Thirty-six adult male Wistar rats were randomly divided into three groups: control (saline treatment), standard treatment (silver sulfadiazine), and test group (Aloe barbadensis Miller ethanol extract). Grade II burn wounds were created using a standardized thermal injury model. Wound healing parameters including wound contraction, epithelialization time, histopathological changes, and biochemical markers were assessed over 21 days.

Results: The Aloe barbadensis Miller ethanol extract group demonstrated significantly enhanced wound healing compared to controls, with accelerated wound contraction (p<0.05), reduced epithelialization time, and improved histological scores. Anti-inflammatory and antioxidant activities were significantly elevated in the treatment group, comparable to standard therapy.